Memory B cell

In immunology, a memory B cell (MBC) is a type of B lymphocyte that forms part of the adaptive immune system. These cells develop within germinal centers of the secondary lymphoid organs. Memory B cells circulate in the blood stream in a quiescent state, sometimes for decades.[1] Their function is to memorize the characteristics of the antigen that activated their parent B cell during initial infection such that if the memory B cell later encounters the same antigen, it triggers an accelerated and robust secondary immune response.[2][3] Memory B cells have B cell receptors (BCRs) on their cell membrane, identical to the one on their parent cell, that allow them to recognize antigen and mount a specific antibody response.[4]


Development and Activation

T cell dependent mechanisms

In a T-cell dependent development pathway, naïve follicular B cells are activated by antigen presenting follicular B helper T cells (TFH) during the initial infection, or primary immune response.[3] Naïve B cells circulate through follicles in secondary lymphoid organs (i.e. spleen and lymph nodes) where they can be activated by a floating foreign peptide brought in through the lymph or by antigen presented by antigen presenting cells (APCs) such as dendritic cells (DCs).[5] B cells may also be activated by binding foreign antigen in the periphery where they then move into the secondary lymphoid organs.[3] A signal transduced by the binding of the peptide to the B cell causes the cells to migrate to the edge of the follicle bordering the T cell area.[5]

The B cells internalize the foreign peptides, break them down, and express them on class II major histocompatibility complexes (MHCII), which are cell surface proteins. Within the secondary lymphoid organs, most of the B cells will enter B-cell follicles where a germinal center will form. Most B cells will eventually differentiate into plasma cells or memory B cells within the germinal center.[3][6] The TFHs that express T cell receptors (TCRs) cognate to the peptide (i.e. specific for the peptide-MHCII complex) at the border of the B cell follicle and T-cell zone will bind to the MHCII ligand. The T cells will then express the CD40 ligand (CD40L) molecule and will begin to secrete cytokines which cause the B cells to proliferate and to undergo class switch recombination, a mutation in the B cell's genetic coding that changes their immunoglobulin type.[7][8] Class switching allows memory B cells to secrete different types of antibodies in future immune responses.[3] The B cells then either differentiate into plasma cells, germinal center B cells, or memory B cells depending on the expressed transcription factors. The activated B cells that expressed the transcription factor Bcl-6 will enter B-cell follicles and undergo germinal center reactions.[7]

Once inside the germinal center, the B cells undergo proliferation, followed by mutation of the genetic coding region of their BCR, a process known as somatic hypermutation.[3] The mutations will either increase or decrease the affinity of the surface receptor for a particular antigen, a progression called affinity maturation. After acquiring these mutations, the receptors on the surface of the B cells (B cell receptors) are tested within the germinal center for their affinity to the current antigen.[9] B cell clones with mutations that have increased the affinity of their surface receptors receive survival signals via interactions with their cognate TFH cells.[2][3][10] The B cells that do not have high enough affinity to receive these survival signals, as well as B cells that are potentially auto-reactive, will be selected against and die through apoptosis.[6] These processes increase variability at the antigen binding sites such that every newly generated B cell has a unique receptor.[11]

After differentiation, memory B cells relocate to the periphery of the body where they will be more likely to encounter antigen in the event of a future exposure.[6][2][3] Many of the circulating B cells become concentrated in areas of the body that have a high likelihood of coming into contact with antigen, such as the Peyer's patch.

The process of differentiation into memory B cells within the germinal center is not yet fully understood.[3] Some researchers hypothesize that differentiation into memory B cells occurs randomly.[6][4] Other hypotheses propose that the transcription factor NF-κB and the cytokine IL-24 are involved in the process of differentiation into memory B cells.[11][3] An additional hypothesis states that the B cells with relatively lower affinity for antigen will become memory B cells, in contrast to B cells with relatively higher affinity that will become plasma cells.

T cell independent mechanisms

Not all B cells present in the body have undergone somatic hypermutations. IgM+ memory B cells that have not undergone class switch recombination demonstrate that memory B cells can be produced independently of the germinal centers.

Primary response

Upon infection with a pathogen, many B cells will differentiate into the plasma cells, also called effector B cells, which produce a first wave of protective antibodies and help clear infection.[6][2] Plasma cells secrete antibodies specific for the pathogens but they cannot respond upon secondary exposure. A fraction of the B cells with BCRs cognate to the antigen differentiate into memory B cells that survive long-term in the body.[12] The memory B cells can maintain their BCR expression and will be able to respond quickly upon secondary exposure. [6]

Secondary response and memory

The memory B cells produced during the primary immune response are specific to the antigen involved during the first exposure. In a secondary response, the memory B cells specific to the antigen or similar antigens will respond.[3] When memory B cells reencounter their specific antigen, they proliferate and differentiate into plasma cells, which then respond to and clear the antigen.[3] The memory B cells that do not differentiate into plasma cells at this point can reenter the germinal centers to undergo further class switching or somatic hypermutation for further affinity maturation.[3] Differentiation of memory B cells into plasma cells is far faster than differentiation by naïve B cells, which allows memory B cells to produce a more efficient secondary immune response.[4] The efficiency and accumulation of the memory B cell response is the foundation for vaccines and booster shots.[4][3]


Memory B cells can survive for decades, which gives them the capacity to respond to multiple exposures to the same antigen.[3] The long-lasting survival is hypothesized to be a result of certain anti-apoptosis genes that are more highly expressed in memory B cells than other subsets of B cells.[6] Additionally, the memory B cell does not need to have continual interaction with the antigen nor with T cells in order to survive long-term.[4]


Memory B cells are typically distinguished by the cell surface marker CD27, although some subsets do not express CD27. Memory B cells that lack CD27 are generally associated with exhausted B cells or certain autoimmune conditions such as HIV, lupus, or rheumatoid arthritis.[2][3]

Because B cells have typically undergone class switching, they can express a range of immunoglobulin molecules. Some specific attributes of particular immunoglobulin molecules are described below:  

The receptor CCR6 is generally a marker of B cells that will eventually differentiate into MBCs. This receptor detects chemokines, which are chemical messengers that allow the B cell to move within the body. Memory B cells may have this receptor to allow them to move out of the germinal center and into the tissues where they have a higher probability of encountering antigen.[6]


Germinal center independent memory B cells

This subset of cells differentiates from activated B cells into memory B cells before entering the germinal center. B cells that have a high level of interaction with TFH within the B cell follicle have a higher propensity of entering the germinal center. The B cells that develop into memory B cells independently from germinal centers likely experience CD40 and cytokine signaling from T cells.[14] Class switching can still occur prior to interaction with the germinal center, while somatic hypermutation only occurs after interaction with the germinal center.[14] The lack of somatic hypermutation is hypothesized to be beneficial; a lower level of affinity maturation means that these memory B cells are less specialized to a specific antigen and may be able to recognize a wider range of antigens.[11][15][4]

T-independent memory B cells

T-independent memory B cells are a subset called B1 cells. These cells generally reside in the peritoneal cavity. When reintroduced to antigen, some of these B1 cells can differentiate into memory B cells without interacting with a T cell.[4] These B cells produce IgM antibodies to help clear infection.[16]

T-bet memory B cells

T-bet B cells are a subset that have been found to express the transcription factor T-bet. T-bet is associated with class switching. T-bet B cells are also thought to be important in immune responses against intracellular bacterial and viral infections.[17]


Vaccines are based on the notion of immunological memory. The preventative injection of a non-pathogenic antigen into the organism allows the body to generate a durable immunological memory. The injection of the antigen leads to an antibody response followed by the production of memory B cells. These memory B cells are promptly reactivated upon infection with the antigen and can effectively protect the organism from disease.[18]

See also


  1. ^ Crotty S, Felgner P, Davies H, Glidewell J, Villarreal L, Ahmed R (November 2003). "Cutting edge: long-term B cell memory in humans after smallpox vaccination" . Journal of Immunology. 171 (10): 4969–73. doi:10.4049/jimmunol.171.10.4969 . PMID 14607890 .
  2. ^ a b c d e Weisel F, Shlomchik M (April 2017). "Memory B Cells of Mice and Humans". Annual Review of Immunology. 35 (1): 255–284. doi:10.1146/annurev-immunol-041015-055531 . PMID 28142324 .
  3. ^ a b c d e f g h i j k l m n o p q Seifert M, Küppers R (December 2016). "Human memory B cells". Leukemia. 30 (12): 2283–2292. doi:10.1038/leu.2016.226 . PMID 27499139 .
  4. ^ a b c d e f g h i j Kurosaki T, Kometani K, Ise W (March 2015). "Memory B cells". Nature Reviews. Immunology. 15 (3): 149–59. doi:10.1038/nri3802 . PMID 25677494 .
  5. ^ a b Garside P, Ingulli E, Merica RR, Johnson JG, Noelle RJ, Jenkins MK (July 1998). "Visualization of specific B and T lymphocyte interactions in the lymph node". Science. 281 (5373): 96–9. doi:10.1126/science.281.5373.96 . PMID 9651253 .
  6. ^ a b c d e f g h Suan D, Sundling C, Brink R (April 2017). "Plasma cell and memory B cell differentiation from the germinal center". Current Opinion in Immunology. Lymphocyte development and activation * Tumour immunology. 45: 97–102. doi:10.1016/j.coi.2017.03.006 . PMID 28319733 .
  7. ^ a b Taylor JJ, Jenkins MK, Pape KA (December 2012). "Heterogeneity in the differentiation and function of memory B cells" . Trends in Immunology. 33 (12): 590–7. doi:10.1016/ . PMC 3505266 . PMID 22920843 .
  8. ^ Phan, Tri Giang; Tangye, Stuart G (2017-04-01). "Memory B cells: total recall" . Current Opinion in Immunology. Lymphocyte development and activation * Tumour immunology. 45: 132–140. doi:10.1016/j.coi.2017.03.005 . ISSN 0952-7915 .
  9. ^ Allman D, Wilmore JR, Gaudette BT (March 2019). "The continuing story of T-cell independent antibodies" . Immunological Reviews. 288 (1): 128–135. doi:10.1111/imr.12754 . PMC 6653682 . PMID 30874357 .
  10. ^ Victora GD, Nussenzweig MC (2012-03-26). "Germinal centers". Annual Review of Immunology. 30 (1): 429–57. doi:10.1146/annurev-immunol-020711-075032 . PMID 22224772 .
  11. ^ a b c Shinnakasu R, Kurosaki T (April 2017). "Regulation of memory B and plasma cell differentiation". Current Opinion in Immunology. Lymphocyte development and activation * Tumour immunology. 45: 126–131. doi:10.1016/j.coi.2017.03.003 . PMID 28359033 .
  12. ^ Gatto D, Brink R (November 2010). "The germinal center reaction". The Journal of Allergy and Clinical Immunology. 126 (5): 898–907, quiz 908-9. doi:10.1016/j.jaci.2010.09.007 . PMID 21050940 .
  13. ^ Seifert M, Küppers R (December 2016). "Human memory B cells". Leukemia. 30 (12): 2283–2292. doi:10.1038/leu.2016.226 . PMID 27499139 .
  14. ^ a b Kurosaki T, Kometani K, Ise W (March 2015). "Memory B cells". Nature Reviews. Immunology. 15 (3): 149–59. doi:10.1038/nri3802 . PMID 25677494 .
  15. ^ Pupovac A, Good-Jacobson KL (April 2017). "An antigen to remember: regulation of B cell memory in health and disease" . Current Opinion in Immunology. Lymphocyte development and activation * Tumour immunology. 45: 89–96. doi:10.1016/j.coi.2017.03.004 . PMC 7126224 . PMID 28319732 .
  16. ^ Montecino-Rodriguez E, Dorshkind K (January 2012). "B-1 B cell development in the fetus and adult" . Immunity. 36 (1): 13–21. doi:10.1016/j.immuni.2011.11.017 . PMC 3269035 . PMID 22284417 .
  17. ^ Knox JJ, Myles A, Cancro MP (March 2019). "T-bet+ memory B cells: Generation, function, and fate" . Immunological Reviews. 288 (1): 149–160. doi:10.1111/imr.12736 . PMC 6626622 . PMID 30874358 .
  18. ^ Dhenni R, Phan TG (July 2020). "The geography of memory B cell reactivation in vaccine-induced immunity and in autoimmune disease relapses". Immunological Reviews. 296 (1): 62–86. doi:10.1111/imr.12862 . PMID 32472583 .

Categories: B cells | Lymphocytes | Human cells | Immunology | Immune system

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